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KMID : 1007520160250010355
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Food Science and Biotechnology
2016 Volume.25 No. 1 p.355 ~ p.360
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Safety of Using Escherichia coli Bacteriophages as a Sanitizing Agent Based on Inflammatory Responses in Rats
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Hwang Ji-Yeon
Kim Jung-Eun
Song Yoon-Jae
Park Jong-Hyun
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Abstract
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Use of bacteriophages as sanitizing agents has received much attention. However, safety in humans is debatable. To determine inflammatory immune responses against bacteriophages, rats were treated with a 8 log plaque-forming cocktail of 5 bacteriophages for pathogenic Escherichia coli per day for 4 weeks. Food consumption, feeding efficiency, and body weight of rats treated with the cocktail were not different from controls. Phages were not detected in the sera of phage-fed rats with no changes in organ weights. Notable changes were not observed upon histopathological examination of the liver, kidney, and spleen. Pro-inflammatory cytokine mRNA expression, except COX-2 (2.4x increase), remained unaffected after treatment with the phage cocktail. No remarkable changes were observed for levels of 12 pro-inflammatory cytokines in sera. Inflammatory responses in rats orally treated with a phage cocktail were not observed. Bacteriophages for E. coli are indicated as immunologically safe in rats.
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KEYWORD
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E. coli bacteriophage, pro-inflammatory cytokine, phage-fed rat
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